Alter and colleagues aimed to overcome limitations in standard laboratory protocols for preparing extracellular vesicles (EVs). They sought to develop an efficient method that could produce consistent EVs with high reproducibility.
The EV preparation method described by the authors for nano plasma membrane vesicles (nPMVs) addresses several limitations of existing EV preparation methods, including efficiency, uniformity, and reproducibility. This method outperforms conventional EV preparation methods in terms of production rate and yield by at least one to two orders of magnitude.
A systematic comparison between nPMVs and native EVs from the same cells revealed biologically relevant differences in proteomes and lipidomes, which can be attributed to the distinct origins and preparation methods of these vesicles.
Read the article here:
#research #lipid #lipidomics #LipidMetabolism #lipotype #lipidome
