New method for molecular profiling of adipose tissue

Henri Deda March 13, 2019

The Paul Langerhans Institute Dresden, a partner of the DZD, and the University Hospital Carl Gustav Carus joined their forces with Lipotype GmbH and developed a new shotgun lipidomics method for the molecular profiling of brown and white adipose tissue. The results of this collaborative work have now been published in the journal ́Molecular Metabolism ́.

To better understand adipose tissue
Adipose tissue consists of more than 99% of storage lipids, mainly triglycerides that are stored within the cell in so-called lipid droplets. Nonetheless, the remaining 0.5-1% of lipids are sufficient to build a metabolically active cell in all its complexity.

Despite the enormous importance of adipose tissue in human health and diseases (like diabetes), no standardized method existed so far, to quantitatively and reproducibly analyze its lipidome. Thus, Paul Langerhans Institute Dresden (PLID), German Center for Diabetes Research (DZD), the Institute for Clinical Chemistry and Laboratory Medicine of the University Hospital Carl Gustav Carus Dresden (IKL) and Lipotype GmbH chose to tackle this urgent need. Their joint vision of an omics-driven clinic, fueled the development of a shotgun lipidomics protocol for adipose tissue.

A new protocol
This new and validated protocol wants to facilitate the systematic molecular profiling of adipose tissue by providing high reproducibility and linear dynamic range for all lipid classes. Besides covering all types of adipose tissue, it can be used for fatty organs, and can be integrated with further omics approaches used in pre-clinical research.

Resources

Press Release: Dresden scientists develop new shotgun lipidomics method for molecular profiling of adipose tissue

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Lipidomics: A Rising Star in Omics Research

Henri Deda February 15, 2019

Technology Networks published a new introductory article on lipidomics last week. They spoke to Dr. Christian Klose about applications in the field of lipid research and to Henri Deda about LEA, the new Lipidomics Excellence Award.

The article in short
The article provides a brief introduction to the research field of lipids: lipidomics. Summarizing recent developments and challenges of the past, granting an overview on today’s lipidomics technology and discussing the broad field of lipidomics applications in detail, the article is a short and entertaining read. Dr Christian Klose, Head of Research and Development at Lipotype, comments on various aspects providing examples for current research and product developments in various industries and academia. To complete the picture of current developments in lipidomics, the motivation to found and organize the Lipidomics Excellence Award finishes off the article.

Lipidomics Excellence Award
LEA, the Lipidomics Excellence Award, has been created to promote, support and strengthen researchers who are eager to contribute to the progress of life sciences through lipidomics. Up to three individuals with ongoing research are awarded with comprehensive research prizes to support their projects. Applications are open to all researchers who are eager to strengthen life sciences through lipidomics. Call for applications is open till March 30, 2019.

LEA is supported by LIPID MAPS, SwissLipids and Lipotype; the media LABO, the Journal of Lipid Research and transkript aligned themselves with LEA too.

Resources

Technology Networks Article: Lipidomics – A Rising Star in “OMICS” Research
Press Release: Lipotype Launches first global Lipidomics Excellence Award

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Lipotype launches first global Lipidomics Excellence Award

Henri Deda January 16, 2019

Lipotype announces LEA, the Lipidomics Excellence Award, for breakthrough lipidomics projects. Call for applications is open till March 30, 2019.

LEA at a glance
LEA has been created to promote, support and strengthen researchers who are eager to contribute to the progress of life sciences through lipidomics.

Up to three individuals with ongoing research are awarded with comprehensive research prizes to support their projects. Applications are open to all researchers who are eager to strengthen life sciences through lipidomics.

The awardees are selected by a panel composed of internationally renown members of academia, each of them recognized as leading experts in their respective fields.

LEA is supported by LIPID MAPS, SwissLipids and Lipotype.

The first prize
The winner of the first prize will receive 55,000 EUR worth of analysis services in order to put their idea to test. The awardee will be presenting their research at an internationally leading experts summit for lipids and lipidomics, the EMBO Workshop “Lipid function in health and disease” in Dresden (Sept 27th – 30th, 2019). A press conference will be held to present the project and formally handover the LEA 2019 trophy.

How to apply
Individual researchers from academia and industry and their ongoing projects can apply for LEA 2019. Applicants are asked to outline their idea for a project or research approach whose results they think can be boosted by a detailed lipid analysis. All applications shall be submitted until March 30, 2019 via the online submission form.

Resources

Press Release: Lipotype Launches first global Lipidomics Excellence Award – ENG
Press Release: Lipotype startet den ersten weltweiten Lipidomics Excellence Award – DEU
Online Submission Form

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Dermal drug development for SLS associated ichthyosis

Henri Deda January 8, 2019

Lipotype put online a new biotech & dermatology customer reference with Aldeyra Therapeutics about lipid analysis for topical dermal drug development for the treatment of ichthyosis associated with SLS.

The project in short
Sjögren-Larsson Syndrome (SLS) is a rare inborn error of aldehyde metabolism characterized by severe skin thickening (ichthyosis), retinal disease, and neurological dysfunction. As of today, no therapy that addresses SLS has been approved. Aldeyra Therapeutics has conducted a Phase 2 randomized, parallel-group, double-blind, vehicle-controlled, multi-center clinical trial of topical dermal reproxalap for the treatment of ichthyosis associated with SLS. The results of the trial demonstrated clinically meaningful and statistically significant improvements of SLS skin symptoms of patients after only eight weeks of treatment. A multi-center Phase 3 clinical trial is ongoing.

What Lipotype did
Lipotype Shotgun Skin Lipidomics provided Aldeyra Therapeutics with lipid analysis of skin samples for identification of relevant lipid biomarkers in the Phase 2 clinical trial. Results of the skin lipidomics analysis delivered evidence for a correlation between clinical effects and those skin lipid biomarkers. Consistent with the clinical activity of reproxalap, significant dermal biomarker changes were noted in reproxalap-treated patients but not vehicle-treated patients. Cholesterol, which is significantly elevated in the skin of SLS patients, was reduced in reproxalap-treated patients more than in vehicle-treated patients.

About Aldeyra Therapeutics
Aldeyra Therapeutics is a biotechnology company devoted to improving lives by inventing, developing, and commercializing next-generation medicines that treat immune-mediated and metabolic diseases. Aldeyra’s lead product candidate, reproxalap (formerly called NS2), is a small molecule RASP (Reactive Aldehyde Species) inhibitor in Phase 3 clinical development for the treatment of dry eye disease, allergic conjunctivitis, noninfectious anterior uveitis, and Sjögren-Larsson syndrome. With its focus on a carefully selected range of diseases, Aldeyra Therapeutics is devoted to improving lives through outstanding innovation.

Find the full customer reference

Customer reference Aldeyra Therapeutics: lipid analysis for topical dermal drug development for the treatment of ichthyosis associated with SLS

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Lipotype presents the awardees of the Lipidomics Forum 2018

Henri Deda November 19, 2018

We proudly present the winners of best poster and best scientific talk award at the Lipidomics Forum 2018.

A short history of the Lipidomics Forum
The Lipidomics Forum is a joint effort of the ISAS Dortmund and the Research Center Borstel. The annual symposium is a series of conferences on lipid biology and lipidomics to provide a platform for international acknowledged scientists, students, and young researchers to interact. It does not only cover current research highlights but also offers tutorials on selected methodical topics.

The Lipidomics Forum has become a home for Lipotype. Besides a shared scientific background, we strive for the same goals such as global scientific excellence and exchange. This is why Lipotype began attending and supporting through awards the conference many years ago.

The awardees
The best poster award was won by Bing Peng (middle). She researches blood platelets and their lipid composition which is critical for platelet integrity and functionality. She could show, that the lipidome changes upon platelet activation. Congrats, Bing Peng!

The best scientific talk award was won by Marcus Höring (right). His work aims at the intestinal lipid metabolism and the influence of gut microbia. He made use of isotope labeled fatty acids to achieve that. Congrat, Marcus Höring!

Ronny Herzog
Dr. Ronny Herzog, Head of Software Development and IT-Infrastructure, and the awardees.

Dominik Schwudke (top left) and Robert Ahrends (bottom left) have been organizing the Lipidomics Forum since its beginning. To say thank you, we gifted both a rafting weekend. We are certain, they will have as much fun as we have with our lipid raft.

Resources

Press Release: Lipotype presents the awardees of the Lipidomics Forum 2018

Abstract book of the Lipidomics Forum 2018

Lipidomics Forum 2018

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Lipotype Shotgun Lipidomics technology in Science journal

Henri Deda October 26, 2018

Every scientist dreams of a report in Science. For us, this dream has just come true. The research project of one of our customers has been published by the iconic journal Science.

The research project in 60 seconds
Cell membranes contain a vast range of distinct lipid species. Their chemical differences influence the physical characteristics of membranes, such as viscosity. Thus, the lipid composition affects biological functions.

To identify the lipids involved, the research group used genetic engineering to modulate the lipid composition of bacteria. They correlated bacterial growth rates with lipid composition. From that they concluded that the level of unsaturated lipids influences cellular respiration, the conversion of nutrients into energy (e.g. ATP).

Further biological research and physical modeling confirmed the results. In the end, they could replicate their findings in yeast mitochondria, the organelle responsible for cellular respiration in animals and plants. Thus, further highlighting the importance of lipidomics in fundamental research.

All science is just as good as the research which precedes it
This publication has been added to the Lipidomics Resource Center, an online tool to empower your lipid research. It features scientific publications, white papers and industry insights. The integrated live full-text search and in-depth tag filter system will help you identify information of your interest.

Lipids are essential to life. Make them essential to your research too!

Resources

Viscous control of cellular respiration by membrane lipid composition – Science, 2018
Itay Budin, Tristan de Rond, Yan Chen, Leanne J G Chan, Christopher J Petzold, Jay D Keasling

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New White Paper “Unlocking the Power of Multiomics”

Henri Deda October 18, 2018

Multiomics approaches harness synergies and eliminate shortcomings to effectively deliver what one omics science alone cannot achieve. Lipotype’s new white paper “Unlocking the Power of Multiomics” highlights why combining genomics with lipidomics makes for a great omics pair.

Genomics for genes, lipidomics for lipids
Being major players in cardiovascular disease research, genomics and lipidomics are perfectly suited for a joint multiomics approach. It matches the capacity to identify genetic predestinations with one or multiple snapshots of the lipid metabolic status.

Linking genotype with phenotype will be critical when it comes to clinical diagnostics and to the goal of identifying the onsets of a disease.

From genotype to phenotype: interconnected disease risk factors
In this study, a large number of SNPs (single-nucleotide polymorphisms) from genomes was drawn. They were then correlated with corresponding lipid species levels from human blood plasma. The blood plasma has been analyzed with Lipotype Shotgun Lipidomics technology. The most important findings are the following.

First, the heritability of lipid species levels was quantified from the SNP correlations. Second, genetic predispositions influencing lipid species levels provide useful information for cardiovascular disease risk predictions. And third, lipidomics yields greater statistical power to identify SNPs with direct roles in lipid metabolism. Lipidomics outperforms traditional lipid panel measures used in clinical routine.

Resources

Lipotype White Paper: Unlocking the Power of Multiomics

Press release: Unlocking the Power of Multiomics – Lipotype releases new Multiomics White Paper

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Press Release: Lipotype and Lipoid celebrate their joint IFSCC 2018 Poster Success

Henri Deda October 9, 2018

Lipotype and Lipoid presented a joint poster featuring the core findings of their skin lipidome research at IFSCC 2018 in Munich. Overwhelmingly positive feedback resulted in placing the poster among the top 10 of all IFSCC 2018 posters.

A successful congress and a great poster
The International Federation of Societies of Cosmetic Chemists is dedicated to international cooperation in cosmetic science and technology. This year’s congress was about “Cosmetics: Science for Beauty and Lifestyle”.

Lipotype and Lipoid chose the IFSCC to present their findings as the congress focuses on exploring what modern society wants from cosmetics and dermatology. This represents both companies’ consumer oriented research best, which was confirmed on-site by the great feedback of those who visited the poster. Ultimately, the IFSCC committee chose to rank the joint poster of Lipotype and Lipoid among the top 10 of all IFSCC 2018 posters.

The project in short
Lipoid conducted this novel study to investigate skin penetration properties of dermal formulations based on hydrogenated phospholipids. For the first time, the quantitative analysis of both exogenous phospholipids and endogenous skin lipids within a single measurement was demonstrated. The results confirm the properties of hydrogenated phospholipids to penetrate and accumulate in upper epidermal layers. Based on these findings, Lipoid will conduct further investigations to improve delivery and fixation of cosmetic actives.

About Lipoid
Founded over 40 years ago, Lipoid is leading in the development and industrial production of high quality lecithin and phospholipids not only for the pharmaceutical, but also for the cosmetic and dietetic industry. Lipoid is the only company worldwide offering the whole range of natural and synthetic phospholipids in industrial scale. Environmental protection and sustainability determines the company’s strategy within all phases of the value chain. This long-standing expertise, in combination with the exceptional quality of the products enables the customers to develop and market innovative products for the benefit of the general public.

Resources

Press release: IFSCC 2018 poster success – Joint poster selected Top 10 of all IFSCC posters

IFSCC 2018 poster: The effect of saturated phospholipids on human skin as assessed by shotgun lipidomic analysis

IFSCC 2018 poster ranking

Customer reference: Lipid analysis for skin penetration properties of saturated phospholipids

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Investigation of photodamaged dry facial skin from different ethnic groups

Henri Deda September 3, 2018

Lipotype put online a new skin lipidomics customer reference with Royal DSM about lipid analysis for the investigation of photodamaged dry facial skin from different ethnic groups.

The project in short
Even though decades of moisturizer development have passed, dry facial skin remains a major concern for consumers. Thus, DSM’s aim was to understand more precisely the underlying biochemistry, particularly of the maturation of the stratum corneum and its relation to facial photodamage, skin pigmentation and ethnicity. In contrast to traditional research of skin analyte composition, a more revolutionary ‘omic’ approach was applied.

What Lipotype did
With its mass spectrometry-based Shotgun Skin Lipidomics technology Lipotype delivered lipid analysis of the ceramidome, the entirety of ceramides. The results precisely highlight changes in ceramide composition and distribution which help to explain the differences in facial skin barrier function, hydration and stratum corneum maturation, but also the skin care need of the differently pigmented skin types.

About DSM
Royal DSM is a purpose-led global science-based company in Nutrition, Health and Sustainable Living. With its innovative business solutions for human nutrition, animal nutrition, personal care and aroma, medical devices, green products and applications, DSM is driving economic prosperity, environmental progress and social advances to create sustainable value. DSM’s purpose is to create brighter lives for people today and generations to come.

Find the full customer reference

Customer reference Royal DSM: lipid analysis for investigation of photodamaged dry facial skin from different ethnic groups

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Got time in September?

Henri Deda August 27, 2018

We will attend numerous national and international events in September. Take your chance to experience Lipotype, study our research and discuss with us!

Meet Lipotype at…

11. – 14. September 2018
16th ESTP, Copenhagen, Denmark
Come and visit us at our booth 7!

The 16th ESTP congress is dedicated to metabolic diseases like diabetes and how they affect neurodegenerative conditions such as Alzheimer’s.

Also, Prof. Kai Simons will hold a keynote presentation on the 12th of September at 9:00 am about “Lipid Rafts, Lipidomics and Health”. We highly recommend to listen to him.

 

18. – 19. September 2018
IFSCC 2018, Munich, Germany
We will visit the IFSCC 2018 and present the poster 216!

Exploring what modern society wants from cosmetics and dermatology – that’s what the IFSCC is all about. Besides presenting our own poster 216 in session 1, DSM’s poster 11 showcases Lipotype Shotgun Lipidomics technology, too.

 

27. – 29. September 2018
7th European Lipidomics Meeting, Leipzig, Germany
We have been invited to give a talk but will exhibit at 7th ELM, too. Visit us at our booth!

The ELM gathers the most prominent lipidomics researchers from all over the world to discuss latest technological developments. We are honored that Dr. Christian Klose has been invited as a speaker. His presentation about “Predicting obesity by machine learning based on human plasma lipidomics data” will take place on September 29th at 10:00 am.

We will staff a booth to discuss with you about latest news in lipids and lipidomics as well as recent research. Besides that, we will sponsor the best poster award.

 

If you want to schedule an individual meeting drop us a line!

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