Tag Technology

HOW CAN OMIC SCIENCE BE IMPROVED?

Kai Simons, founder and CEO of Lipotype, has been following the development of omic science for decades. His lifelong experience as well as his proficiency in lipids and lipidomics has led him to a detailed review.

The published article “How can Omic Science be improved?” critically analyzes the shortcomings of modern omic science, which yet could not fulfill its promise of a paradigm shift in medicine and biology. Bot, scientific evolution and present challenges of this field of research are taken into account to picture the overall view.

While the article itself focuses on lipidomics, Kai Simon’s field of expertise, all lessons learned can be applied generally to omic science.


Read the full article here:
How can Omic Science be improved? – Proteomics Journal, 2018
Kai Simons

Skin lipidomics: a tool for understanding skin aging at the molecular level

Lipotype published in SOFW Journal about skin lipidomics to better understand skin aging

The lipid composition of human skin is essential for its function; however the simultaneous quantification of a wide range of skin lipids, belonging to both stratum corneum and sebum, is not trivial. Recently we developed a new, “shotgun” mass spectrometry-based method characterized by a broad coverage of lipids, absolute quantitation, high reproducibility and high-throughput providing comprehensive insight into skin lipid composition [1].

We applied this method in a large-scale study of natural lipid variability of human skin. In this study we analyzed samples from 104 individuals of both sexes of different ages. We discovered gradual changes in the skin lipidome correlated with age but no sex-specific differences. Our novel method enables detailed and broad insights into skin lipidomes, both in terms of lipidome coverage and sample number. Therefore, skin shotgun lipidomics has the potential to become useful in studies related to skin aging and anti-aging product claims support.

Read more: 
Click her to read the full article in SOFW Journal


Learn more about Lipotype Skin Lipidomics
 Download our White Paper

[1] Large-scale human skin lipidomics by quantitative, high-throughput shotgun mass spectrometry – Scientific Reports, 2017
Tomasz Sadowski, Christian Klose, Mathias J. Gerl, Anna Wójcik-MaciejewiczRonny HerzogKai SimonsAdam Reich, Michal A. Surma

Release of White Paper: Big data lipidomics for lipid biomarker identification

Lipidomics is the large-scale investigation of lipids in biological systems. Venturing into the analysis of large dataset, with potential thousands of lipidomes, by advanced multiparametric statistical approaches is a challenging endeavour. We established algorithms and a full set of methods tailored to quantitative lipid data. This allows to perform statistical analysis and enrichment analysis in order to identify lipid biomarkers. In our recently published white paper a cohort of healthy subjects is compared with a cohort of diseased subjects. The result is a lipid signature that discriminates healthy from disease. Those could then potentially be of use for disease stratification or diagnosis by means of predictive modelling (machine learning). In this white paper, we will guide you through the data analysis process for lipid biomarkers.   Read more


Learn more about Big Data Lipidomics and get the White Paper.


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Lipidomics paves the way to groundbreaking scientific discoveries

VIB’s Technology Watch team has been active for almost a decade, continuously analyzing the potential of emerging technologies and mediating researchers’ access to them. By expanding VIB’s network of technology suppliers, purchasing licenses and negotiating business partnerships, the Tech Watch team enables VIB scientists to have privileged access to cutting-edge scientific innovations. One example comes in the form of a powerful lipidomics research tool made available to VIB scientists through a collaboration with Lipotype, a Max-Planck spin-off and key service provider. Since 2015, Lipotype technology has been a crucial factor in several major breakthroughs in multiple life sciences domains.

The benefits of a dedicated lipidomics collaborator
Lipidomics, or the analysis, identification and quantification of lipids, has important applications in basic and clinical research, as well as in the nutrition, cosmetics and personalized healthcare industries. Using Lipotype’s Shotgun Lipidomics Technology, researchers can rapidly analyze large samples at reasonable prices, benefiting from the ultra-broad coverage and absolute quantification of lipids enabled by this technology.

Mark Veugelers (VIB Tech Watch): “The Tech Watch team, in close collaboration with VIB group leaders, is constantly on the lookout for new technologies that can have a major impact on our research. The Lipidomics services Lipotype offers is a good example. But identification of a novel technology with potential isn’t enough to produce breakthrough results. Key in the process is the hands-on experience of VIB scientists when evaluating the potential of these novel technology opportunities.

Oliver Uecke (Lipotype): “Tech Watch at VIB is a fantastic instrument to support technology transfer.  It’s a win-win situation for all parties involved: VIB researchers get access to innovative technologies early on, while technology providers have the tech watch team as single entry point to address the VIB collective of researchers. VIB profits from providing their researchers with means to generate cutting edge research results and with an improved basis for licensing and spin-off creation.”

Better tech integration, impactful results
After just two years of collaboration, VIB scientists working with Lipotype have published three papers in high-impact academic journals. This demonstrates the fact that partnerships between VIB and specialized tech service providers and the integration of new innovations into VIB research leads to more and faster discoveries.

Prof. Wim Annaert (VIB-KU Leuven): “Lipotype’s Shotgun technology was critical to our recent paper on a new approach to analyzing subcellular dysfunction. Our next challenge is now to integrate lipidomics with proteomics data to better understand what goes on at the level of a single subcellular compartment for instance in a disease context.”
(relevant publication: A novel approach to analyze lysosomal dysfunctions through subcellular proteomics and lipidomics: the case of NPC1 deficiency, Tharkeshwar et al., Scientific Reports 2017)

Prof. Patrik Verstreken (VIB-KU Leuven): “My team recently made groundbreaking discoveries related to mitochondrial defects in Parkinson’s Disease. Tech Watch – and access to Lipotype tech in particular – were essential to these findings.”
(relevant publication: Cardiolipin promotes electron transport between ubiquinone and complex I to rescue PINK1 deficiency, Vos et al., J Cell Biol 2017)

Prof. Rose Goodchild (VIB-KU Leuven): “Tech Watch funding significantly boosted our confidence in collaborating with Lipotype for our work on cellular lipid metabolism. Lipidomics is a key emerging technology that we don’t have in-house, and our access to it – especially when it comes to molecular and cellular research – adds huge value to our projects.”
(relevant publication: Torsins Are Essential Regulators of Cellular Lipid Metabolism, Gonzalez et al., Developmental Cell 2016)

Read the full press release here
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 Download the Microsoft Word Version

 


About Lipotype

Lipotype is a spin-off company from the Kai Simons and Andrej Shevchenko labs of the world-renowned Max-Planck-Institute of Molecular Cell Biology and Genetics in Dresden, Germany. Drawing on many years of cutting edge research experience, Lipotype delivers comprehensive, absolutely quantitative lipid analysis services for clinical and biological samples on a high-throughput scale.

Lipotype offers high quality lipid analysis services for a wide range of customers and applications including biomarker identification for clinical researchers, pharma and biotech companies, functional food development for the food industry, as well as for the small-scale profiling needs of academic researchers.


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Novel skin lipidomics for cosmetic and dermatological applications

Lipotype GmbH, a German Max-Planck spin-off company, launched its novel Lipotype Shotgun Skin Lipidomics Technology and offers lipid analysis services for applications such as cosmetic claim support, topical drug development, efficacy testing for dermatological pharmacology and the cosmetic industry.

High-throughput skin lipidomics with ultra-broad fully quantitative coverage
Until recently, investigating skin lipidomes was not a trivial task. On one hand, the analytical method needs to have coverage broad enough to encompass the variety of skin lipids, and a throughput allowing for statistically relevant studies. On the other hand the method should be compatible with a convenient sampling technique such as tape stripping. These prerequisites are now met in the Lipotype Shotgun Skin Lipidomics Technology platform as explained by Prof. Kai Simons, CEO of Lipotype: “We offer unprecedented throughput of hundreds of samples, absolutely quantified with broad coverage of ceramides, triglycerides and other lipids, and this is all available for samples collected by super-easy tape stripping.” The development of Lipotype Skin Lipidomics was supported by the German Central Innovation Programme for SMEs.

Applications of skin lipidomics
With tools such as Lipotype Skin Lipidomics it is now easy to investigate how the healthy skin lipidome is composed, how it changes in diseases or upon intervention with a drug or a cosmetic product. This lipidomic data can be used for cosmetic claim support, topical drug development and efficacy testing. “We are excited about new possibilities to include skin lipidomics in the product development process of cosmetics companies as well as for biomarker identification for dermatology”, says Dr. Oliver Uecke, Head of Business Development and Finance at Lipotype GmbH.

Read the full press release here
Open Adobe PDF Version
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“HIGHLIGHTS” of our novel Lipotype Shotgun Skin Lipidomics Technology

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Lipotype and Nestle Institute of Health Sciences publish paper about the Lipotype shotgun lipidomics platform

An automated shotgun lipidomics platform for high throughput, comprehensive,
and quantitative analysis of blood plasma intact lipids

Blood plasma has gained protagonism in lipidomics studies due to its availability, uncomplicated collection and preparation, and informative readout of physiological status. At the same time, it is also technically challenging to analyze due to its complex lipid composition affected by many factors, which can hamper the throughput and/or lipidomics coverage.

To tackle these issues, we developed a comprehensive, high throughput, and quantitative mass spectrometry-based shotgun lipidomics platform for blood plasma lipid analyses. The main hallmarks of this technology are (i) it is comprehensive, covering 22 quantifiable different lipid classes encompassing more than 200 lipid species; (ii) it is amenable to high-throughput, with less than 5min acquisition time allowing the complete analysis of 200 plasma samples per day; (iii) it achieves absolute quantification, by inclusion of internal standards for every lipid class measured; (iv) it is highly reproducible, achieving an average coefficient of variation of <10% (intra-day), approx. 10% (inter-day), and approx. 15% (inter-site) for most lipid species; (v) it is easily transferable allowing the direct comparison of data acquired in different sites.

Moreover, we thoroughly assessed the influence of blood stabilization with different anticoagulants and freeze-thaw cycles to exclude artifacts generated by sample preparation.

Access to the full paper

 


 

Reference

A. Surma, R. Herzog, A. Vasilj, C. Klose, N. Christinat, D. Morin-Rivron, K. Simons, M. Masoodi, and J. L. Sampaio: “An Automated Shotgun Lipidomics Platform for High Throughput, Comprehensive, and Quantitative Analysis of Blood Plasma Intact Lipids.”.
 European Journal of Lipid Science and Technology (2015) Volume 117, Issue 10 (doi:10.1002/ejlt.201500145)