Standard B. subtilis Subcellular
Lipidomics

Optional Reports

Correlation Analysis
The Correlation Analysis Report includes an advanced data analysis and contains results of up to 8 pairwise Correlation Analyses. The delivery time for the Correlation Analysis Report will be 3 weeks after the Standard and Extended Report are delivered to the customer. There is one revision round of the Correlation Analysis Report included which can prolong the delivery time. Within this package a continuous parameter describing samples (e.g., age, a clinical parameter, protein expression level etc.) is correlated with amounts of lipid (sub-)species/classes and other data, and includes

• Correlation analysis with lipid (sub-)species:
  Contains histogram of correlation estimates, their p-values and individual graphical presentation of significant correlations (scatter plots).
• Correlation analysis with lipid classes and fatty acids:
  Contains sums within classes measured as subspecies. Contains histogram of correlation estimates, p-values and individual graphical presentation of significant correlations (scatter plots).

• Pathway enrichment analysis (optional):
  Contains an analysis based on the lipid classes of significantly correlating lipid (sub-)species.

Cohort Comparison
The Cohort Comparison Report includes an advanced data analysis and contains results of up to 8 pairwise cohort comparisons. The delivery time for the Cohort Comparison Report will be 3 weeks after the Standard and Extended Report are delivered to the customer. There is one revision round of the Cohort Comparison included which can prolong the delivery time. This package compares two specified groups each containing multiple samples, and includes

• Cohort Comparison of lipid (sub-)species:
  Contains significance tests, graphical presentation of the result of the pairwise comparison (volcano plot) and individual graphical presentation of significant hits (box plots).
• Cohort Comparison of lipid classes and fatty acids:
  Contains sums within classes measured as subspecies. Contains significance tests and individual graphical presentation of significant hits (box plots).
• Cohort Comparison of lipid features:
  (Total length, total double bond and hydroxylation sums within a class.) Contains significance tests and individual graphical presentation of significant hits (box plots).

• Pathway enrichment analysis (optional):
  Contains an analysis based on the lipid classes of the significant lipid (sub-)species hits.

LipotypeZoom
Interactive Data Visualization with LipotypeZoom is available free of charge for 6 months. LipotypeZoom displays the content of the extended report and is internet-accessible via browser. The selection of LipotypeZoom gives consent to Lipotype GmbH to upload the results.

LipotypeZoom PRO
LipotypeZoom PRO is a browser-based interactive tool specifically designed for analysing lipidomics data. It simplifies and streamlines the process and allows you to gain insights from your lipidomics datasets with ease. Using principal component analysis (PCA) and orthogonal partial least squares – discriminant analysis (OPLS-DA), LipotypeZoom PRO gives you a quick overview of your entire project and enables detailed analysis with statistical tests (t-test, Wilcoxon) in paired and unpaired variants. To decipher complex lipid patterns from various lipid structure features such as saturation, chain length and hydroxylation, LipotypeZoom PRO provides you with a fully-fledged enrichment analysis that can be tailored exactly to your needs. Whether you’re interested in KEGG-pathways, Rhea-reactions or feature analysis, we’ve got you covered. What’s more, you can also export plots to share your results with others to speed up decision-making. LipotypeZoom PRO is accessible via internet browser for 6 months after delivery of the results. The selection of LipotypeZoom PRO gives consent to Lipotype GmbH to upload the results.