Detailed Product Description
Analytical Procedure
Each sample is extracted, acquired and analyzed once. We recommend including multiple sample replicates in order to obtain statistically meaningful data.
Analytical Approach
Untargeted high-resolution shotgun lipidomics-generated data analyzed using LipotypeXplorer, Lipotype’s proprietary software for identifying all measurable lipids using the molecular fragmentation query language (MFQL) and thus unimpeded by the traditional reliance on predefined spectral databases.
Quality Assurance
Each analysis includes internal blank and quality control reference samples to assess and improve the technical quality of the analysis. The technical variation of an analytical run assessed from quality control reference samples is provided within the Report. Please note: No further information or data (especially also including individualized data) on blank and quality control reference samples and/or their analysis will be provided to the customer.
Description of Results
Results are fully quantitative and expressed in absolute values – pmol per sample volume and mol%. The absolute quantification is achieved using lipid class-specific internal standards. Lipotype will provide the data on sample volume analyzed and molar amounts of lipid molecules per sample. Please note: Lipotype will not and shall not be obliged to provide any Lipotype background intellectual property (including Lipotype know-how) or any data incorporating such Lipotype background intellectual property, notably no raw data will be provided.
Structural Details
Lipids are reported at the “species” level, meaning that total length, unsaturation and hydroxylations are calculated by summing the properties of the individual fatty acid moieties within a given molecule – e.g. PC 34:2;0 denotes a PC molecule with a total carbon length of 34, 2 double bonds and 0 hydroxylations.
Delivery Time
The Results will be delivered via email within 6 weeks after receiving the samples prepared according to sample preparation guidelines as well as all information regarding the samples and analysis to be performed. The delivery date of samples to Lipotype will be discussed and agreed between the customer and Lipotype. Lipotype’s obligation to perform under this quotation shall be suspended as long as and to the extent Lipotype is not able to fully and unrestrainedly use its facilities and personnel due to injunctions, restraints, directions or recommendations (individual or public) by any local, national or international governmental or non-governmental institutions (“Authority Measures”), regardless of whether such Authority Measures affect Lipotype, its directors, employees, agents or subcontractors directly or indirectly. The selection of Turbo Delivery reduces the delivery time for standard and extended reports of this analysis package to 3 weeks turbo analysis.