The Paul Langerhans Institute Dresden, a partner of the DZD, and the University Hospital Carl Gustav Carus joined their forces with Lipotype GmbH and developed a new shotgun lipidomics method for the molecular profiling of brown and white adipose tissue. The results of this collaborative work have now been published in the journal ́Molecular Metabolism ́.
To better understand adipose tissue
Adipose tissue consists of more than 99% of storage lipids, mainly triglycerides that are stored within the cell in so-called lipid droplets. Nonetheless, the remaining 0.5-1% of lipids are sufficient to build a metabolically active cell in all its complexity.
Despite the enormous importance of adipose tissue in human health and diseases (like diabetes), no standardized method existed so far, to quantitatively and reproducibly analyze its lipidome. Thus, Paul Langerhans Institute Dresden (PLID), German Center for Diabetes Research (DZD), the Institute for Clinical Chemistry and Laboratory Medicine of the University Hospital Carl Gustav Carus Dresden (IKL) and Lipotype GmbH chose to tackle this urgent need. Their joint vision of an omics-driven clinic, fueled the development of a shotgun lipidomics protocol for adipose tissue.
A new protocol
This new and validated protocol wants to facilitate the systematic molecular profiling of adipose tissue by providing high reproducibility and linear dynamic range for all lipid classes. Besides covering all types of adipose tissue, it can be used for fatty organs, and can be integrated with further omics approaches used in pre-clinical research.