CASE STUDY

ER-Plasma membrane contact site lipids

Yale University, School of Medicine
De Camilli Lab

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The De Camilli Lab at the Yale School of Medicine focuses on mechanisms underlying the dynamics and traffic of intracellular membranes. The De Camilli Lab was interested in determining the impact of lipid transfer proteins (LTPs) – in particular those that act at ER-plasma membrane contact sites – on the lipid composition of the plasma membrane.

With its Shotgun Lipidomics technology, Lipotype delivered its one-of-a-kind, detailed, quantitative information on lipid structure and composition of control and knockout cells for selected LTPs and of their purified plasma membranes.

These observations provided new insight into mechanisms controlling lipid homeostasis at the plasma membrane. This fruitful collaboration between the De Camilli Lab and Lipotype demonstrates the power of lipid analysis in advanced cell biology research.

About the De Camilli Lab/Yale School of Medicine

We study mechanisms underlying the dynamics and traffic of intracellular membranes, with emphasis on membrane transport reactions involved in neurotransmission. A major long-term goal is to advance the understanding of nervous system function in health and disease. We also exploit the unique structural and functional features of synapses to learn about general principles in membrane biology.

 

Reference

Control of plasma membrane lipid homeostasis by the extended synaptotagmins. – Nature cell biology, 2016 USA

Y Saheki, X Bian, CM Schauder, Y Sawaki, MA Surma, C Klose, F Pincet, KM Reinisch, P De Camilli