CASE STUDY

Lysosomal storage disorder in HeLa cells

Wim Annaert Lab
VIB Center for the Biology of Disease, KU Leuven

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The laboratory of professor Wim Annaert at the Flanders Institute for Biotechnology (VIB) Center for the Biology of Disease, KU Leuven focuses on understanding the molecular biology of membrane transport in a disease-related context with a major focus on Alzheimer’s disease.

The Annaert Lab has developed superparamagnetic iron oxide nanopartilces (SPIONs) for the isolation of ultrapure plasma membranes and lysosomes allowing subsequent high resolution profi ling of their biomolecular compositon. With its Shotgun Lipidomics technology, Lipotype delivered its one-of-a-kind, detailed, quantitative information on lipid structure and composition of cell culture samples and purifi ed cellular organelles. This fruitful collaboration between the Annaert Lab and Lipotype demonstrates the power of lipid analysis in cell biological research.

About the Wim Annaert Lab/ VIB Center for the Biology of Disease, KU Leuven

The Wim Annaert laboratory is focused on understanding the molecular biology of membrane transport in a disease-related context covering mainly Alzheimer’s disease.

Reference

A novel approach to analyze lysosomal dysfunctions through subcellular proteomics and lipidomics: the case of NPC1 deficiency – Scientific Reports, 2017

Arun K. Tharkeshwar, Jesse Trekker, Wendy Vermeire, Jarne Pauwels, Ragna Sannerud, David A. Priestman, Danielle te Vruchte, Katlijn Vints, Pieter Baatsen, Jean-Paul Decuypere, Huiqi Lu, Shaun Martin, Peter Vangheluwe, Johannes V. Swinnen, Liesbet Lagae, Francis Impens, Frances M. Platt, Kris Gevaert, Wim Annaert